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One reason GHK is interesting is that it binds copper in a way that may influence how copper behaves in tissues

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1c,d) and was previously suggested as a candidate for a potential activator site, first, because its positive charge might stabilize or bind GSH as GS , and, second, because of its evolutionary conserved position that is equivalent to the GSH-interacting catalytic residue of glutathione transferases 3

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Adjust the concentration of SSA in the sample solution to 0.5-1% with ddH2O before the assay